Construct: shRNA TRCN0000366641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172700.2-1691s21c1
DNA Barcode:
CTGGATGCTCTTCCGGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmpste24 (230709)
Gene Description:
zinc metallopeptidase, STE24
Transcript:
RefSeq NM_172700.2 (CURRENT)
Match location:
Position 1691 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATGCTCTTCCGGTTATT
Hairpin Sequence:
5'-CCGG-CTGGATGCTCTTCCGGTTATT-CTCGAG-AATAACCGGAAGAGCATCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATGCTCTTCCGGTTATTCTCGAGAATAACCGGAAGAGCATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATGCTCTTCCGGTTATTCTCGAGAATAACCGGAAGAGCATCCAG-3'

Other clones with same target sequence:

(none)