Construct: shRNA TRCN0000366749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133786.3-358s21c1
DNA Barcode:
TCGTCTCATGATAACTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smc4 (70099)
Gene Description:
structural maintenance of chromosomes 4
Transcript:
RefSeq NM_133786.3 (CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGTCTCATGATAACTCATAT
Hairpin Sequence:
5'-CCGG-TCGTCTCATGATAACTCATAT-CTCGAG-ATATGAGTTATCATGAGACGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGTCTCATGATAACTCATATCTCGAGATATGAGTTATCATGAGACGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGTCTCATGATAACTCATATCTCGAGATATGAGTTATCATGAGACGA-3'

Other clones with same target sequence:

(none)