Construct: shRNA TRCN0000366981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145431.2-1207s21c1
DNA Barcode:
GCCTCCTTCGACAAGTCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nle1 (217011)
Gene Description:
notchless homolog 1 (Drosophila)
Transcript:
RefSeq NM_145431.2 (CURRENT)
Match location:
Position 1207 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCTTCGACAAGTCTATC
Hairpin Sequence:
5'-CCGG-GCCTCCTTCGACAAGTCTATC-CTCGAG-GATAGACTTGTCGAAGGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCTTCGACAAGTCTATCCTCGAGGATAGACTTGTCGAAGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCTTCGACAAGTCTATCCTCGAGGATAGACTTGTCGAAGGAGGC-3'

Other clones with same target sequence:

(none)