Construct: shRNA TRCN0000367061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163793.1-4113s21c1
DNA Barcode:
GTTCGTTGTAAAGAGTGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C530008M17Rik (320827)
Gene Description:
RIKEN cDNA C530008M17 gene
Transcript:
RefSeq NM_001163793.1 (CURRENT)
Match location:
Position 4113 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGTTGTAAAGAGTGTATT
Hairpin Sequence:
5'-CCGG-GTTCGTTGTAAAGAGTGTATT-CTCGAG-AATACACTCTTTACAACGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGTTGTAAAGAGTGTATTCTCGAGAATACACTCTTTACAACGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGTTGTAAAGAGTGTATTCTCGAGAATACACTCTTTACAACGAAC-3'

Other clones with same target sequence:

(none)