Construct: shRNA TRCN0000367198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029909.1-719s21c1
DNA Barcode:
GCCTAACTTTAGCGGAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C330018D20Rik (77422)
Gene Description:
RIKEN cDNA C330018D20 gene
Transcript:
RefSeq NM_029909.1 (CURRENT)
Match location:
Position 719 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAACTTTAGCGGAGTAAA
Hairpin Sequence:
5'-CCGG-GCCTAACTTTAGCGGAGTAAA-CTCGAG-TTTACTCCGCTAAAGTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAACTTTAGCGGAGTAAACTCGAGTTTACTCCGCTAAAGTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAACTTTAGCGGAGTAAACTCGAGTTTACTCCGCTAAAGTTAGGC-3'

Other clones with same target sequence:

(none)