Construct: shRNA TRCN0000367262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029685.1-1048s21c1
DNA Barcode:
GGGCTCTTCTACCCGTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700113H08Rik (76640)
Gene Description:
RIKEN cDNA 1700113H08 gene
Transcript:
RefSeq NM_029685.1 (CURRENT)
Match location:
Position 1048 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCTCTTCTACCCGTTTATT
Hairpin Sequence:
5'-CCGG-GGGCTCTTCTACCCGTTTATT-CTCGAG-AATAAACGGGTAGAAGAGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCTCTTCTACCCGTTTATTCTCGAGAATAAACGGGTAGAAGAGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCTCTTCTACCCGTTTATTCTCGAGAATAAACGGGTAGAAGAGCCC-3'

Other clones with same target sequence:

(none)