Construct: shRNA TRCN0000367272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164275.1-481s21c1
DNA Barcode:
CCTTTCCCTCAGCAACTTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prrg1 (546336)
Gene Description:
proline rich Gla (G-carboxyglutamic acid) 1
Transcript:
RefSeq NM_001164275.1 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCCCTCAGCAACTTAAC
Hairpin Sequence:
5'-CCGG-CCTTTCCCTCAGCAACTTAAC-CTCGAG-GTTAAGTTGCTGAGGGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCCCTCAGCAACTTAACCTCGAGGTTAAGTTGCTGAGGGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCCCTCAGCAACTTAACCTCGAGGTTAAGTTGCTGAGGGAAAGG-3'

Other clones with same target sequence:

(none)