Construct: shRNA TRCN0000367325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164258.1-489s21c1
DNA Barcode:
TGCTACCTCTAGATGTCTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrra1 (446101)
Gene Description:
X-ray radiation resistance associated 1
Transcript:
RefSeq NM_001164258.1 (CURRENT)
Match location:
Position 489 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTACCTCTAGATGTCTTTC
Hairpin Sequence:
5'-CCGG-TGCTACCTCTAGATGTCTTTC-CTCGAG-GAAAGACATCTAGAGGTAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTACCTCTAGATGTCTTTCCTCGAGGAAAGACATCTAGAGGTAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTACCTCTAGATGTCTTTCCTCGAGGAAAGACATCTAGAGGTAGCA-3'

Other clones with same target sequence:

(none)