Construct: shRNA TRCN0000367352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028474.1-2434s21c1
DNA Barcode:
TATCCGGTTCATCGTGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptchd4 (627626)
Gene Description:
patched domain containing 4
Transcript:
RefSeq NM_028474.1 (CURRENT)
Match location:
Position 2434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCCGGTTCATCGTGTTTAA
Hairpin Sequence:
5'-CCGG-TATCCGGTTCATCGTGTTTAA-CTCGAG-TTAAACACGATGAACCGGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCCGGTTCATCGTGTTTAACTCGAGTTAAACACGATGAACCGGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCCGGTTCATCGTGTTTAACTCGAGTTAAACACGATGAACCGGATA-3'

Other clones with same target sequence:

(none)