Construct: shRNA TRCN0000367393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005608.2-389s21c1
DNA Barcode:
ATGAGCAGGACACAGACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRCAP (5790)
Gene Description:
protein tyrosine phosphatase receptor type C associated protein
Transcript:
RefSeq NM_005608.2 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGCAGGACACAGACTATG
Hairpin Sequence:
5'-CCGG-ATGAGCAGGACACAGACTATG-CTCGAG-CATAGTCTGTGTCCTGCTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGCAGGACACAGACTATGCTCGAGCATAGTCTGTGTCCTGCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGCAGGACACAGACTATGCTCGAGCATAGTCTGTGTCCTGCTCAT-3'

Other clones with same target sequence:

(none)