Construct: shRNA TRCN0000367463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000553.4-3762s21c1
DNA Barcode:
CAGCGTCTTGCCGATCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WRN (7486)
Gene Description:
WRN RecQ like helicase
Transcript:
RefSeq NM_000553.4 (NON-CURRENT)
Match location:
Position 3762 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCGTCTTGCCGATCAATAT
Hairpin Sequence:
5'-CCGG-CAGCGTCTTGCCGATCAATAT-CTCGAG-ATATTGATCGGCAAGACGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCGTCTTGCCGATCAATATCTCGAGATATTGATCGGCAAGACGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCGTCTTGCCGATCAATATCTCGAGATATTGATCGGCAAGACGCTG-3'

Other clones with same target sequence:

(none)