Construct: shRNA TRCN0000367680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032595.3-2748s21c1
DNA Barcode:
ACAAGCACCTGCAGATCAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R9B (84687)
Gene Description:
protein phosphatase 1 regulatory subunit 9B
Transcript:
RefSeq NM_032595.3 (NON-CURRENT)
Match location:
Position 2748 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCACCTGCAGATCAAAC
Hairpin Sequence:
5'-CCGG-ACAAGCACCTGCAGATCAAAC-CTCGAG-GTTTGATCTGCAGGTGCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCACCTGCAGATCAAACCTCGAGGTTTGATCTGCAGGTGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCACCTGCAGATCAAACCTCGAGGTTTGATCTGCAGGTGCTTGT-3'

Other clones with same target sequence:

(none)