Construct: shRNA TRCN0000367709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005458.6-563s21c1
DNA Barcode:
CATCCGTCACATCCATCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABBR2 (9568)
Gene Description:
gamma-aminobutyric acid type B receptor subunit 2
Transcript:
RefSeq NM_005458.6 (NON-CURRENT)
Match location:
Position 563 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCGTCACATCCATCATTG
Hairpin Sequence:
5'-CCGG-CATCCGTCACATCCATCATTG-CTCGAG-CAATGATGGATGTGACGGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCGTCACATCCATCATTGCTCGAGCAATGATGGATGTGACGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCGTCACATCCATCATTGCTCGAGCAATGATGGATGTGACGGATG-3'

Other clones with same target sequence:

(none)