Construct: shRNA TRCN0000367845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000916.3-2232s21c1
DNA Barcode:
TCCTGACCTCAAAGTGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OXTR (5021)
Gene Description:
oxytocin receptor
Transcript:
RefSeq NM_000916.3 (CURRENT)
Match location:
Position 2232 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGACCTCAAAGTGTATTT
Hairpin Sequence:
5'-CCGG-TCCTGACCTCAAAGTGTATTT-CTCGAG-AAATACACTTTGAGGTCAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGACCTCAAAGTGTATTTCTCGAGAAATACACTTTGAGGTCAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGACCTCAAAGTGTATTTCTCGAGAAATACACTTTGAGGTCAGGA-3'

Other clones with same target sequence:

(none)