Construct: shRNA TRCN0000367872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020226.3-1533s21c1
DNA Barcode:
AGAACCTTGAAGCCTACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM8 (56978)
Gene Description:
PR/SET domain 8
Transcript:
RefSeq NM_020226.3 (CURRENT)
Match location:
Position 1533 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACCTTGAAGCCTACATAA
Hairpin Sequence:
5'-CCGG-AGAACCTTGAAGCCTACATAA-CTCGAG-TTATGTAGGCTTCAAGGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACCTTGAAGCCTACATAACTCGAGTTATGTAGGCTTCAAGGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACCTTGAAGCCTACATAACTCGAGTTATGTAGGCTTCAAGGTTCT-3'

Other clones with same target sequence:

(none)