Construct: shRNA TRCN0000367980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010890.3-1833s21c1
DNA Barcode:
GGATTCTTACCGGAGGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nedd4 (17999)
Gene Description:
neural precursor cell expressed, developmentally down-regulated 4
Transcript:
RefSeq NM_010890.3 (CURRENT)
Match location:
Position 1833 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATTCTTACCGGAGGATTAT
Hairpin Sequence:
5'-CCGG-GGATTCTTACCGGAGGATTAT-CTCGAG-ATAATCCTCCGGTAAGAATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATTCTTACCGGAGGATTATCTCGAGATAATCCTCCGGTAAGAATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATTCTTACCGGAGGATTATCTCGAGATAATCCTCCGGTAAGAATCC-3'

Other clones with same target sequence:

(none)