Construct: shRNA TRCN0000368023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010718.3-2561s21c1
DNA Barcode:
GTGAAATGACAGTAGCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Limk2 (16886)
Gene Description:
LIM motif-containing protein kinase 2
Transcript:
RefSeq NM_010718.3 (NON-CURRENT)
Match location:
Position 2561 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAATGACAGTAGCGATTT
Hairpin Sequence:
5'-CCGG-GTGAAATGACAGTAGCGATTT-CTCGAG-AAATCGCTACTGTCATTTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAATGACAGTAGCGATTTCTCGAGAAATCGCTACTGTCATTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAATGACAGTAGCGATTTCTCGAGAAATCGCTACTGTCATTTCAC-3'

Other clones with same target sequence:

(none)