Construct: shRNA TRCN0000368473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153838.3-2536s21c1
DNA Barcode:
GCTTTAAGGAGCATGATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRF4 (221393)
Gene Description:
adhesion G protein-coupled receptor F4
Transcript:
RefSeq NM_153838.3 (NON-CURRENT)
Match location:
Position 2536 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAAGGAGCATGATTTAT
Hairpin Sequence:
5'-CCGG-GCTTTAAGGAGCATGATTTAT-CTCGAG-ATAAATCATGCTCCTTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAAGGAGCATGATTTATCTCGAGATAAATCATGCTCCTTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAAGGAGCATGATTTATCTCGAGATAAATCATGCTCCTTAAAGC-3'

Other clones with same target sequence:

(none)