Construct: shRNA TRCN0000368502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000914.3-671s21c1
DNA Barcode:
TGATCTCCATAGATTACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OPRM1 (4988)
Gene Description:
opioid receptor mu 1
Transcript:
RefSeq NM_000914.3 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCTCCATAGATTACTATA
Hairpin Sequence:
5'-CCGG-TGATCTCCATAGATTACTATA-CTCGAG-TATAGTAATCTATGGAGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCTCCATAGATTACTATACTCGAGTATAGTAATCTATGGAGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCTCCATAGATTACTATACTCGAGTATAGTAATCTATGGAGATCA-3'

Other clones with same target sequence:

(none)