Construct: shRNA TRCN0000368534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170606.2-4502s21c1
DNA Barcode:
ATGACCCATTAGCTGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KMT2C (58508)
Gene Description:
lysine methyltransferase 2C
Transcript:
RefSeq NM_170606.2 (NON-CURRENT)
Match location:
Position 4502 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACCCATTAGCTGATATTT
Hairpin Sequence:
5'-CCGG-ATGACCCATTAGCTGATATTT-CTCGAG-AAATATCAGCTAATGGGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACCCATTAGCTGATATTTCTCGAGAAATATCAGCTAATGGGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACCCATTAGCTGATATTTCTCGAGAAATATCAGCTAATGGGTCAT-3'

Other clones with same target sequence:

  1. TRCN0000238935