Construct: shRNA TRCN0000368780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011079.2-1662s21c1
DNA Barcode:
TCAACCTCTGGTACAGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phkg1 (18682)
Gene Description:
phosphorylase kinase gamma 1
Transcript:
RefSeq NM_011079.2 (NON-CURRENT)
Match location:
Position 1662 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAACCTCTGGTACAGCTAAA
Hairpin Sequence:
5'-CCGG-TCAACCTCTGGTACAGCTAAA-CTCGAG-TTTAGCTGTACCAGAGGTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAACCTCTGGTACAGCTAAACTCGAGTTTAGCTGTACCAGAGGTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAACCTCTGGTACAGCTAAACTCGAGTTTAGCTGTACCAGAGGTTGA-3'

Other clones with same target sequence:

(none)