Construct: shRNA TRCN0000368916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003369.3-2403s21c1
DNA Barcode:
ACTTAACCCTTTGTGATAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UVRAG (7405)
Gene Description:
UV radiation resistance associated
Transcript:
RefSeq NM_003369.3 (NON-CURRENT)
Match location:
Position 2403 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTAACCCTTTGTGATAATG
Hairpin Sequence:
5'-CCGG-ACTTAACCCTTTGTGATAATG-CTCGAG-CATTATCACAAAGGGTTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTAACCCTTTGTGATAATGCTCGAGCATTATCACAAAGGGTTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTAACCCTTTGTGATAATGCTCGAGCATTATCACAAAGGGTTAAGT-3'

Other clones with same target sequence:

(none)