Construct: shRNA TRCN0000369045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000465.2-571s21c1
DNA Barcode:
TGGTTTAGCCCTCGAAGTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BARD1 (580)
Gene Description:
BRCA1 associated RING domain 1
Transcript:
RefSeq NM_000465.2 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTTTAGCCCTCGAAGTAAG
Hairpin Sequence:
5'-CCGG-TGGTTTAGCCCTCGAAGTAAG-CTCGAG-CTTACTTCGAGGGCTAAACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTTTAGCCCTCGAAGTAAGCTCGAGCTTACTTCGAGGGCTAAACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTTTAGCCCTCGAAGTAAGCTCGAGCTTACTTCGAGGGCTAAACCA-3'

Other clones with same target sequence:

(none)