Construct: shRNA TRCN0000369114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016252.3-14962s21c1
DNA Barcode:
TGGATAAATCACTGCTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BIRC6 (57448)
Gene Description:
baculoviral IAP repeat containing 6
Transcript:
RefSeq NM_016252.3 (CURRENT)
Match location:
Position 14962 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATAAATCACTGCTATATA
Hairpin Sequence:
5'-CCGG-TGGATAAATCACTGCTATATA-CTCGAG-TATATAGCAGTGATTTATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATAAATCACTGCTATATACTCGAGTATATAGCAGTGATTTATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATAAATCACTGCTATATACTCGAGTATATAGCAGTGATTTATCCA-3'

Other clones with same target sequence:

(none)