Construct: shRNA TRCN0000369176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005057.2-1051s21c1
DNA Barcode:
ACCCATCATAGCATCCATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBBP5 (5929)
Gene Description:
RB binding protein 5, histone lysine methyltransferase complex subunit
Transcript:
RefSeq NM_005057.2 (NON-CURRENT)
Match location:
Position 1051 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCATCATAGCATCCATTTC
Hairpin Sequence:
5'-CCGG-ACCCATCATAGCATCCATTTC-CTCGAG-GAAATGGATGCTATGATGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCATCATAGCATCCATTTCCTCGAGGAAATGGATGCTATGATGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCATCATAGCATCCATTTCCTCGAGGAAATGGATGCTATGATGGGT-3'

Other clones with same target sequence:

(none)