Construct: shRNA TRCN0000369188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004089.3-351s21c1
DNA Barcode:
TTTCCGTTAAGCTGGACAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSC22D3 (1831)
Gene Description:
TSC22 domain family member 3
Transcript:
RefSeq NM_004089.3 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCCGTTAAGCTGGACAACA
Hairpin Sequence:
5'-CCGG-TTTCCGTTAAGCTGGACAACA-CTCGAG-TGTTGTCCAGCTTAACGGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCCGTTAAGCTGGACAACACTCGAGTGTTGTCCAGCTTAACGGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCCGTTAAGCTGGACAACACTCGAGTGTTGTCCAGCTTAACGGAAA-3'

Other clones with same target sequence:

(none)