Construct: shRNA TRCN0000369313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182588.2-5226s21c1
DNA Barcode:
ACGTCTTGCTGCAGTTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD4 (285190)
Gene Description:
RANBP2 like and GRIP domain containing 4
Transcript:
RefSeq NM_182588.2 (CURRENT)
Match location:
Position 5226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCTTGCTGCAGTTCATTT
Hairpin Sequence:
5'-CCGG-ACGTCTTGCTGCAGTTCATTT-CTCGAG-AAATGAACTGCAGCAAGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCTTGCTGCAGTTCATTTCTCGAGAAATGAACTGCAGCAAGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCTTGCTGCAGTTCATTTCTCGAGAAATGAACTGCAGCAAGACGT-3'

Other clones with same target sequence:

(none)