Construct: shRNA TRCN0000369611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144658.3-6060s21c1
DNA Barcode:
ACTAAATGAGCGGCTAATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK11 (139818)
Gene Description:
dedicator of cytokinesis 11
Transcript:
RefSeq NM_144658.3 (CURRENT)
Match location:
Position 6060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAAATGAGCGGCTAATTAA
Hairpin Sequence:
5'-CCGG-ACTAAATGAGCGGCTAATTAA-CTCGAG-TTAATTAGCCGCTCATTTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAAATGAGCGGCTAATTAACTCGAGTTAATTAGCCGCTCATTTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAAATGAGCGGCTAATTAACTCGAGTTAATTAGCCGCTCATTTAGT-3'

Other clones with same target sequence:

  1. TRCN0000265580