Construct: shRNA TRCN0000369635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039706.2-3278s21c1
DNA Barcode:
CTTTGGAAAGGCTACCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP69 (79846)
Gene Description:
cilia and flagella associated protein 69
Transcript:
RefSeq NM_001039706.2 (NON-CURRENT)
Match location:
Position 3278 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGAAAGGCTACCAATTT
Hairpin Sequence:
5'-CCGG-CTTTGGAAAGGCTACCAATTT-CTCGAG-AAATTGGTAGCCTTTCCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGAAAGGCTACCAATTTCTCGAGAAATTGGTAGCCTTTCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGAAAGGCTACCAATTTCTCGAGAAATTGGTAGCCTTTCCAAAG-3'

Other clones with same target sequence:

(none)