Construct: shRNA TRCN0000369726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014050.2-801s21c1
DNA Barcode:
TATGTGCGGTCTCCAATTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL42 (28977)
Gene Description:
mitochondrial ribosomal protein L42
Transcript:
RefSeq NM_014050.2 (NON-CURRENT)
Match location:
Position 801 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGTGCGGTCTCCAATTTAG
Hairpin Sequence:
5'-CCGG-TATGTGCGGTCTCCAATTTAG-CTCGAG-CTAAATTGGAGACCGCACATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGTGCGGTCTCCAATTTAGCTCGAGCTAAATTGGAGACCGCACATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGTGCGGTCTCCAATTTAGCTCGAGCTAAATTGGAGACCGCACATA-3'

Other clones with same target sequence:

(none)