Construct: shRNA TRCN0000369741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014519.2-1838s21c1
DNA Barcode:
GCCTTTAGTCAAAGCTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF232 (7775)
Gene Description:
zinc finger protein 232
Transcript:
RefSeq NM_014519.2 (NON-CURRENT)
Match location:
Position 1838 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTAGTCAAAGCTCATAT
Hairpin Sequence:
5'-CCGG-GCCTTTAGTCAAAGCTCATAT-CTCGAG-ATATGAGCTTTGACTAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTAGTCAAAGCTCATATCTCGAGATATGAGCTTTGACTAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTAGTCAAAGCTCATATCTCGAGATATGAGCTTTGACTAAAGGC-3'

Other clones with same target sequence:

(none)