Construct: shRNA TRCN0000369772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024871.2-591s21c1
DNA Barcode:
GGAAGAAGTTCACTCCTAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP6D1 (79929)
Gene Description:
MAP6 domain containing 1
Transcript:
RefSeq NM_024871.2 (NON-CURRENT)
Match location:
Position 591 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAGAAGTTCACTCCTAACC
Hairpin Sequence:
5'-CCGG-GGAAGAAGTTCACTCCTAACC-CTCGAG-GGTTAGGAGTGAACTTCTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAGAAGTTCACTCCTAACCCTCGAGGGTTAGGAGTGAACTTCTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAGAAGTTCACTCCTAACCCTCGAGGGTTAGGAGTGAACTTCTTCC-3'

Other clones with same target sequence:

(none)