Construct: shRNA TRCN0000369884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005552.4-1367s21c1
DNA Barcode:
CAAGTATGAAGAAGTAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLC1 (3831)
Gene Description:
kinesin light chain 1
Transcript:
RefSeq NM_005552.4 (CURRENT)
Match location:
Position 1367 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTATGAAGAAGTAGAATA
Hairpin Sequence:
5'-CCGG-CAAGTATGAAGAAGTAGAATA-CTCGAG-TATTCTACTTCTTCATACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTATGAAGAAGTAGAATACTCGAGTATTCTACTTCTTCATACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTATGAAGAAGTAGAATACTCGAGTATTCTACTTCTTCATACTTG-3'

Other clones with same target sequence:

(none)