Construct: shRNA TRCN0000369931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018417.3-2109s21c1
DNA Barcode:
TCCCTATTTCTCGGGAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCY10 (55811)
Gene Description:
adenylate cyclase 10
Transcript:
RefSeq NM_018417.3 (NON-CURRENT)
Match location:
Position 2109 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTATTTCTCGGGAGATTT
Hairpin Sequence:
5'-CCGG-TCCCTATTTCTCGGGAGATTT-CTCGAG-AAATCTCCCGAGAAATAGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTATTTCTCGGGAGATTTCTCGAGAAATCTCCCGAGAAATAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTATTTCTCGGGAGATTTCTCGAGAAATCTCCCGAGAAATAGGGA-3'

Other clones with same target sequence:

(none)