Construct: shRNA TRCN0000369962
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021111.2-3147s21c1
- DNA Barcode:
- CCGCCAATATTAGTAGGATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RECK (8434)
- Gene Description:
- reversion inducing cysteine rich protein with kazal motifs
- Transcript:
- RefSeq NM_021111.2 (NON-CURRENT)
- Match location:
- Position 3147 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000369962)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGCCAATATTAGTAGGATTT
- Hairpin Sequence:
-
5'-CCGG-CCGCCAATATTAGTAGGATTT-CTCGAG-AAATCCTACTAATATTGGCGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGCCAATATTAGTAGGATTTCTCGAGAAATCCTACTAATATTGGCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGCCAATATTAGTAGGATTTCTCGAGAAATCCTACTAATATTGGCGG-3'
Other clones with same target sequence:
(none)
