Construct: shRNA TRCN0000369983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014765.2-463s21c1
DNA Barcode:
GTTACTAGCTCAAGGTGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOMM20 (9804)
Gene Description:
translocase of outer mitochondrial membrane 20
Transcript:
RefSeq NM_014765.2 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTACTAGCTCAAGGTGAATA
Hairpin Sequence:
5'-CCGG-GTTACTAGCTCAAGGTGAATA-CTCGAG-TATTCACCTTGAGCTAGTAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTACTAGCTCAAGGTGAATACTCGAGTATTCACCTTGAGCTAGTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTACTAGCTCAAGGTGAATACTCGAGTATTCACCTTGAGCTAGTAAC-3'

Other clones with same target sequence:

(none)