Construct: shRNA TRCN0000369997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007242.4-225s21c1
DNA Barcode:
AGATACCAATGGTGCTGTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX19B (11269)
Gene Description:
DEAD-box helicase 19B
Transcript:
RefSeq NM_007242.4 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000369997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATACCAATGGTGCTGTTGT
Hairpin Sequence:
5'-CCGG-AGATACCAATGGTGCTGTTGT-CTCGAG-ACAACAGCACCATTGGTATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATACCAATGGTGCTGTTGTCTCGAGACAACAGCACCATTGGTATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATACCAATGGTGCTGTTGTCTCGAGACAACAGCACCATTGGTATCT-3'

Other clones with same target sequence:

(none)