Construct: shRNA TRCN0000370187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030629.2-262s21c1
DNA Barcode:
TCTTACTGCAGGCTGCCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMIP (80790)
Gene Description:
c-Maf inducing protein
Transcript:
RefSeq NM_030629.2 (NON-CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTACTGCAGGCTGCCAATA
Hairpin Sequence:
5'-CCGG-TCTTACTGCAGGCTGCCAATA-CTCGAG-TATTGGCAGCCTGCAGTAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTACTGCAGGCTGCCAATACTCGAGTATTGGCAGCCTGCAGTAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTACTGCAGGCTGCCAATACTCGAGTATTGGCAGCCTGCAGTAAGA-3'

Other clones with same target sequence:

(none)