Construct: shRNA TRCN0000370321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006534.2-4510s21c1
DNA Barcode:
TGACACTGCACTAGGATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCOA3 (8202)
Gene Description:
nuclear receptor coactivator 3
Transcript:
RefSeq NM_006534.2 (NON-CURRENT)
Match location:
Position 4510 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACACTGCACTAGGATTATT
Hairpin Sequence:
5'-CCGG-TGACACTGCACTAGGATTATT-CTCGAG-AATAATCCTAGTGCAGTGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACACTGCACTAGGATTATTCTCGAGAATAATCCTAGTGCAGTGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACACTGCACTAGGATTATTCTCGAGAATAATCCTAGTGCAGTGTCA-3'

Other clones with same target sequence:

(none)