Construct: shRNA TRCN0000370365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021229.3-1467s21c1
DNA Barcode:
ACCTGTCACTTCGACGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTN4 (59277)
Gene Description:
netrin 4
Transcript:
RefSeq NM_021229.3 (NON-CURRENT)
Match location:
Position 1467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGTCACTTCGACGTTAAT
Hairpin Sequence:
5'-CCGG-ACCTGTCACTTCGACGTTAAT-CTCGAG-ATTAACGTCGAAGTGACAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGTCACTTCGACGTTAATCTCGAGATTAACGTCGAAGTGACAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGTCACTTCGACGTTAATCTCGAGATTAACGTCGAAGTGACAGGT-3'

Other clones with same target sequence:

(none)