Construct: shRNA TRCN0000370409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005629.3-748s21c1
DNA Barcode:
ATTACCTGGTCAAGTCCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A8 (6535)
Gene Description:
solute carrier family 6 member 8
Transcript:
RefSeq NM_005629.3 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTACCTGGTCAAGTCCTTTA
Hairpin Sequence:
5'-CCGG-ATTACCTGGTCAAGTCCTTTA-CTCGAG-TAAAGGACTTGACCAGGTAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTACCTGGTCAAGTCCTTTACTCGAGTAAAGGACTTGACCAGGTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTACCTGGTCAAGTCCTTTACTCGAGTAAAGGACTTGACCAGGTAAT-3'

Other clones with same target sequence:

(none)