Construct: shRNA TRCN0000370569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013437.3-3089s21c1
DNA Barcode:
ACTAGATGTTAAGGTTGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRP12 (29967)
Gene Description:
LDL receptor related protein 12
Transcript:
RefSeq NM_013437.3 (NON-CURRENT)
Match location:
Position 3089 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAGATGTTAAGGTTGTTAA
Hairpin Sequence:
5'-CCGG-ACTAGATGTTAAGGTTGTTAA-CTCGAG-TTAACAACCTTAACATCTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAGATGTTAAGGTTGTTAACTCGAGTTAACAACCTTAACATCTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAGATGTTAAGGTTGTTAACTCGAGTTAACAACCTTAACATCTAGT-3'

Other clones with same target sequence:

(none)