Construct: shRNA TRCN0000370638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015409.3-3998s21c1
DNA Barcode:
GCCTTATACGAGGACGTTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EP400 (57634)
Gene Description:
E1A binding protein p400
Transcript:
RefSeq NM_015409.3 (NON-CURRENT)
Match location:
Position 3998 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTATACGAGGACGTTATC
Hairpin Sequence:
5'-CCGG-GCCTTATACGAGGACGTTATC-CTCGAG-GATAACGTCCTCGTATAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTATACGAGGACGTTATCCTCGAGGATAACGTCCTCGTATAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTATACGAGGACGTTATCCTCGAGGATAACGTCCTCGTATAAGGC-3'

Other clones with same target sequence:

(none)