Construct: shRNA TRCN0000370670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138799.2-525s21c1
DNA Barcode:
CAGGCCCAATGATGATCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MBOAT2 (129642)
Gene Description:
membrane bound O-acyltransferase domain containing 2
Transcript:
RefSeq NM_138799.2 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCCCAATGATGATCATTA
Hairpin Sequence:
5'-CCGG-CAGGCCCAATGATGATCATTA-CTCGAG-TAATGATCATCATTGGGCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCCCAATGATGATCATTACTCGAGTAATGATCATCATTGGGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCCCAATGATGATCATTACTCGAGTAATGATCATCATTGGGCCTG-3'

Other clones with same target sequence:

  1. TRCN0000217116
  2. TRCN0000248024