Construct: shRNA TRCN0000370701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015688.1-3184s21c1
DNA Barcode:
GATCTGAGTTCCAGGATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM184B (27146)
Gene Description:
family with sequence similarity 184 member B
Transcript:
RefSeq NM_015688.1 (NON-CURRENT)
Match location:
Position 3184 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTGAGTTCCAGGATTAAT
Hairpin Sequence:
5'-CCGG-GATCTGAGTTCCAGGATTAAT-CTCGAG-ATTAATCCTGGAACTCAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTGAGTTCCAGGATTAATCTCGAGATTAATCCTGGAACTCAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTGAGTTCCAGGATTAATCTCGAGATTAATCCTGGAACTCAGATC-3'

Other clones with same target sequence:

(none)