Construct: shRNA TRCN0000370724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052997.2-3384s21c1
DNA Barcode:
ACTGAAAGAGGAATCATTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD30A (91074)
Gene Description:
ankyrin repeat domain 30A
Transcript:
RefSeq NM_052997.2 (CURRENT)
Match location:
Position 3384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGAAAGAGGAATCATTAAC
Hairpin Sequence:
5'-CCGG-ACTGAAAGAGGAATCATTAAC-CTCGAG-GTTAATGATTCCTCTTTCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGAAAGAGGAATCATTAACCTCGAGGTTAATGATTCCTCTTTCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGAAAGAGGAATCATTAACCTCGAGGTTAATGATTCCTCTTTCAGT-3'

Other clones with same target sequence:

(none)