Construct: shRNA TRCN0000370817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001144869.1-444s21c1
DNA Barcode:
GGCTAGACGATCGCAAGATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIPT2 (387787)
Gene Description:
lipoyl(octanoyl) transferase 2
Transcript:
RefSeq NM_001144869.1 (NON-CURRENT)
Match location:
Position 444 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTAGACGATCGCAAGATCT
Hairpin Sequence:
5'-CCGG-GGCTAGACGATCGCAAGATCT-CTCGAG-AGATCTTGCGATCGTCTAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTAGACGATCGCAAGATCTCTCGAGAGATCTTGCGATCGTCTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTAGACGATCGCAAGATCTCTCGAGAGATCTTGCGATCGTCTAGCC-3'

Other clones with same target sequence:

(none)