Construct: shRNA TRCN0000370925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015171.2-1774s21c1
DNA Barcode:
TTGGACTATCTGACAAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO6 (23214)
Gene Description:
exportin 6
Transcript:
RefSeq NM_015171.2 (NON-CURRENT)
Match location:
Position 1774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000370925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGACTATCTGACAAGTAAA
Hairpin Sequence:
5'-CCGG-TTGGACTATCTGACAAGTAAA-CTCGAG-TTTACTTGTCAGATAGTCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGACTATCTGACAAGTAAACTCGAGTTTACTTGTCAGATAGTCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGACTATCTGACAAGTAAACTCGAGTTTACTTGTCAGATAGTCCAA-3'

Other clones with same target sequence:

(none)