Construct: shRNA TRCN0000371027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016218.2-1845s21c1
DNA Barcode:
TAAATGTGAAGCCGTGAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLK (51426)
Gene Description:
DNA polymerase kappa
Transcript:
RefSeq NM_016218.2 (NON-CURRENT)
Match location:
Position 1845 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAATGTGAAGCCGTGAATAA
Hairpin Sequence:
5'-CCGG-TAAATGTGAAGCCGTGAATAA-CTCGAG-TTATTCACGGCTTCACATTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAATGTGAAGCCGTGAATAACTCGAGTTATTCACGGCTTCACATTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAATGTGAAGCCGTGAATAACTCGAGTTATTCACGGCTTCACATTTA-3'

Other clones with same target sequence:

(none)