Construct: shRNA TRCN0000371051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040065.1-2159s21c1
DNA Barcode:
GAGTGACTCGGGAAGTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATA31A2 (642265)
Gene Description:
family with sequence similarity 75, member A2
Transcript:
RefSeq NM_001040065.1 (NON-CURRENT)
Match location:
Position 2159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGACTCGGGAAGTGATTT
Hairpin Sequence:
5'-CCGG-GAGTGACTCGGGAAGTGATTT-CTCGAG-AAATCACTTCCCGAGTCACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGACTCGGGAAGTGATTTCTCGAGAAATCACTTCCCGAGTCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGACTCGGGAAGTGATTTCTCGAGAAATCACTTCCCGAGTCACTC-3'

Other clones with same target sequence:

(none)